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mouse heart lysate  (Novus Biologicals)


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    Novus Biologicals mouse heart lysate
    Mouse Heart Lysate, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+heart+tissue+lysate/Mouse+Heart+Whole+Tissue+Lysate+(Adult+Whole+Normal)/pm35860280-60-33-36
    Average 92 stars, based on 5 article reviews
    mouse heart lysate - by Bioz Stars, 2026-09
    92/100 stars

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    Related Articles

    Western Blot:

    Article Title: Members of the Toll-like receptor family of innate immunity pattern-recognition receptors are abundant in the male rat reproductive tract.
    Article Snippet: Mouse RAW 264.7 þ LPS/IFN-c Abelson-transformed macrophage whole cell lysate (sc-24767), SW480 human colorectal adenocarcinoma whole cell lysate (sc-2219), and Daudi cell (Burkitt lymphoma) whole cell lysate (sc2415) were purchased from Santa Cruz Biotechnology.Mouse RAW 264.7 þ LPS/IFN-c Abelson-transformed macrophage whole cell lysate (sc-24767), SW480 human colorectal adenocarcinoma whole cell lysate (sc-2219), and Daudi cell (Burkitt lymphoma) whole cell lysate (sc2415) were purchased from Santa Cruz Biotechnology.. Mouse heart tissue lysate (40102) and Ramos cell lysate (40175) was from Imgenex.. RNA Isolation and RT-PCR Analysis of TLR mRNARNA Isolation and RT-PCR Analysis of TLR mRNA

    Control:

    Article Title: Members of the Toll-like receptor family of innate immunity pattern-recognition receptors are abundant in the male rat reproductive tract.
    Article Snippet: Mouse RAW 264.7 þ LPS/IFN-c Abelson-transformed macrophage whole cell lysate (sc-24767), SW480 human colorectal adenocarcinoma whole cell lysate (sc-2219), and Daudi cell (Burkitt lymphoma) whole cell lysate (sc2415) were purchased from Santa Cruz Biotechnology.Mouse RAW 264.7 þ LPS/IFN-c Abelson-transformed macrophage whole cell lysate (sc-24767), SW480 human colorectal adenocarcinoma whole cell lysate (sc-2219), and Daudi cell (Burkitt lymphoma) whole cell lysate (sc2415) were purchased from Santa Cruz Biotechnology.. Mouse heart tissue lysate (40102) and Ramos cell lysate (40175) was from Imgenex.. RNA Isolation and RT-PCR Analysis of TLR mRNARNA Isolation and RT-PCR Analysis of TLR mRNA

    Positive Control:

    Article Title: Members of the Toll-like receptor family of innate immunity pattern-recognition receptors are abundant in the male rat reproductive tract.
    Article Snippet: Mouse RAW 264.7 þ LPS/IFN-c Abelson-transformed macrophage whole cell lysate (sc-24767), SW480 human colorectal adenocarcinoma whole cell lysate (sc-2219), and Daudi cell (Burkitt lymphoma) whole cell lysate (sc2415) were purchased from Santa Cruz Biotechnology.Mouse RAW 264.7 þ LPS/IFN-c Abelson-transformed macrophage whole cell lysate (sc-24767), SW480 human colorectal adenocarcinoma whole cell lysate (sc-2219), and Daudi cell (Burkitt lymphoma) whole cell lysate (sc2415) were purchased from Santa Cruz Biotechnology.. Mouse heart tissue lysate (40102) and Ramos cell lysate (40175) was from Imgenex.. RNA Isolation and RT-PCR Analysis of TLR mRNARNA Isolation and RT-PCR Analysis of TLR mRNA

    Transformation Assay:

    Article Title: Members of the Toll-like receptor family of innate immunity pattern-recognition receptors are abundant in the male rat reproductive tract.
    Article Snippet: Mouse RAW 264.7 þ LPS/IFN-c Abelson-transformed macrophage whole cell lysate (sc-24767), SW480 human colorectal adenocarcinoma whole cell lysate (sc-2219), and Daudi cell (Burkitt lymphoma) whole cell lysate (sc2415) were purchased from Santa Cruz Biotechnology.Mouse RAW 264.7 þ LPS/IFN-c Abelson-transformed macrophage whole cell lysate (sc-24767), SW480 human colorectal adenocarcinoma whole cell lysate (sc-2219), and Daudi cell (Burkitt lymphoma) whole cell lysate (sc2415) were purchased from Santa Cruz Biotechnology.. Mouse heart tissue lysate (40102) and Ramos cell lysate (40175) was from Imgenex.. RNA Isolation and RT-PCR Analysis of TLR mRNARNA Isolation and RT-PCR Analysis of TLR mRNA



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    FIG. 2. Immunoblot analysis of TLR proteins in male reproductive organs. Aliquots (100 lg) of cytoplasmic protein extracts were separated by PAGE, electroblotted, and blots probed with anti-TLR antibodies followed by enhanced chemiluminescence detection using Pierce Super- Signal West Pico or Pierce SuperSignal West Femto (*F) substrate. Representative results are shown (n ¼ 3–5 rats). þ, control rat <t>tissue</t> extracts from spleen, TLRs 1–7; lung, TLR8; and small intestine, TLR9. þ Ext, positive-control whole cell lysates used were Raw 264 Abelson transformed macrophages (TLRs 1, 2–6, and 8–10); SW480 colorectal adenocarcinoma (TLR2); Daudi cell extract (TLR7); Ramos cell <t>lysate</t> (TLR10) and <t>mouse</t> <t>heart</t> whole cell lysate (TLR11). Blots were stripped and reprobed with anti-actin monoclonal antibody to detect actin as a loading control. Representative results are shown (n ¼ 3–5 rats) for blots exposed to film for the same length of time when using equivalent chemiluminescent substrate.
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    FIG. 2. Immunoblot analysis of TLR proteins in male reproductive organs. Aliquots (100 lg) of cytoplasmic protein extracts were separated by PAGE, electroblotted, and blots probed with anti-TLR antibodies followed by enhanced chemiluminescence detection using Pierce Super- Signal West Pico or Pierce SuperSignal West Femto (*F) substrate. Representative results are shown (n ¼ 3–5 rats). þ, control rat tissue extracts from spleen, TLRs 1–7; lung, TLR8; and small intestine, TLR9. þ Ext, positive-control whole cell lysates used were Raw 264 Abelson transformed macrophages (TLRs 1, 2–6, and 8–10); SW480 colorectal adenocarcinoma (TLR2); Daudi cell extract (TLR7); Ramos cell lysate (TLR10) and mouse heart whole cell lysate (TLR11). Blots were stripped and reprobed with anti-actin monoclonal antibody to detect actin as a loading control. Representative results are shown (n ¼ 3–5 rats) for blots exposed to film for the same length of time when using equivalent chemiluminescent substrate.

    Journal: Biology of reproduction

    Article Title: Members of the Toll-like receptor family of innate immunity pattern-recognition receptors are abundant in the male rat reproductive tract.

    doi: 10.1095/biolreprod.106.059410

    Figure Lengend Snippet: FIG. 2. Immunoblot analysis of TLR proteins in male reproductive organs. Aliquots (100 lg) of cytoplasmic protein extracts were separated by PAGE, electroblotted, and blots probed with anti-TLR antibodies followed by enhanced chemiluminescence detection using Pierce Super- Signal West Pico or Pierce SuperSignal West Femto (*F) substrate. Representative results are shown (n ¼ 3–5 rats). þ, control rat tissue extracts from spleen, TLRs 1–7; lung, TLR8; and small intestine, TLR9. þ Ext, positive-control whole cell lysates used were Raw 264 Abelson transformed macrophages (TLRs 1, 2–6, and 8–10); SW480 colorectal adenocarcinoma (TLR2); Daudi cell extract (TLR7); Ramos cell lysate (TLR10) and mouse heart whole cell lysate (TLR11). Blots were stripped and reprobed with anti-actin monoclonal antibody to detect actin as a loading control. Representative results are shown (n ¼ 3–5 rats) for blots exposed to film for the same length of time when using equivalent chemiluminescent substrate.

    Article Snippet: Mouse heart tissue lysate (40102) and Ramos cell lysate (40175) was from Imgenex.

    Techniques: Western Blot, Control, Positive Control, Transformation Assay